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KMID : 0613820220320121005
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Journal of Life Science
2022 Volume.32 No. 12 p.1005 ~ p.1012
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Comparison of Fecal Microbiota between Birth and Weaning of Halla Horses Using 16S rRNA Gene Amplicon Sequencing
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Lee Jong-An
Kang Young-Jun
Choi Jae-Young
Shin Sang-Min
Shin Moon-Cheol
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Abstract
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This study was conducted to investigate the taxonomic composition and diversity of fecal microbiota between birth and weaning stages of Halla horses using 16S rRNA gene amplicon sequencing analysis. Proteobacteria (35.7%) and Firmicutes (45.6%) were identified as the most common phylum in birth and weaning, respectively. Escherichia (19.7%) and Clostridium (14.0%) were observed as the most dominant genus in birth, and Fibrobacter (6.6%) was the highest in weaning. The results of α-diversity showed that the richness and evenness in microbial communities were statistically significant (p<0.001) in birth and weaning. The results of β-diversity indicated that the birth and weaning stages were
clearly divided into two groups at the genus and species levels. Permutational multivariate analysis of variance (PERMANOVA) showed that the microbiota composition differences between birth and weaning were statistically significant (q<0.001). A linear discriminant analysis effect (LEfSe) was performed to select taxonomic makers between the birth and weaning stages. On the genus level, Escherichia, Bacteroides, Clostridium, and Methylobacterium were relatively abundant at birth, whereas Fibrobacter was more abundant at weaning. We expect that this research can be utilized as basic data in the identification of microbial communities involved in disease prevention and nutrient absorption in Halla horses.
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KEYWORD
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Diversity, fecal microbiota, Halla horses, taxonomic composition
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